What is a retroviral plasmid?
γ-Retrovirus (gamma-retrovirus) is an RNA virus comprised of its genome and several structural and enzymatic proteins, including reverse transcriptase and integrase. Once in a target cell, the virus uses the reverse transcriptase in tow to generate a DNA provirus.
What are plat e cells?
A potent retrovirus packaging cell line named Platinum-E (Plat-E) was generated based on the 293T cell line. Plat-E is superior to existing packaging cell lines regarding efficiency, stability and safety.
What is an ecotropic virus?
[ ē′kə-trŏp′ĭk, -trō′pĭk, ĕk′ə- ] n. An oncornavirus that does not produce disease in its natural host, but does replicate in tissue culture cells derived from the host species.
What are packaging cells?
The cells are called “packaging cells” because they “package” the retroviral vector (which carries the gene of interest) into a delivery vehicle (the retroviral particles). Miller summarizes our present state-of-knowledge of these “packaging” cells. Advancement of Biomimetic Nanoparticles for Targeted Drug Delivery.
What are packaging cell lines?
Packaging Lines Currently, the most efficient gene transfer methodologies harness the capacity of engineered viruses, such as retroviruses, to bypass natural cellular barriers to exogenous nucleic acid uptake.
What is the difference between lentivirus and retrovirus?
Lentiviruses are a subtype of retrovirus. The main difference between lentiviruses and standard retroviruses from an experimental standpoint is lentiviruses are capable of infecting non-dividing and actively dividing cell types, whereas standard retroviruses can only infect mitotically active cell types.
How do packaging cell lines work?
Packaging cell lines can produce replication-competent helper viruses as a result of recombination between the defective helper virus used to construct the cells and an introduced retroviral vector, by recombination of the defective helper and/or retroviral vector with endogenous sequences, or by activation of …
What is the difference between a virus and retrovirus?
A retrovirus is a virus that uses RNA as its genetic material. When a retrovirus infects a cell, it makes a DNA copy of its genome that is inserted into the DNA of the host cell. There are a variety of different retroviruses that cause human diseases such as some forms of cancer and AIDS.