How do you calculate ascorbic acid using titration?
Calculation. For standard solution Mass Ascorbic acid = Mole iodine × Volume of iodine × 176.12 = 0.00231 × 45 × 176.12 = 91.54 mg Initially, the amount of Ascorbic acid was taken for 100mg and therefore for total amount of ascorbic acid i.e. 250 mg the ratio stands out to be 2.5 (250/100).
What is the use of iodine in estimation of ascorbic acid?
Ascorbic acid determination was carried out by iodine titration. When iodine is added to a starch solution, it reacts to produce a purple color. However, if there is any vitamin C in the solution, it “neutralizes” the iodine, preventing the formation of the purple color.
What happens when ascorbic acid reacts with iodine?
When ascorbic acid reacts with iodine, the ascorbic acid is oxidized (looses electrons) and the iodine is reduced (gains electrons). As long as the solution contains ascorbic acid, the iodine is used up in a rapid. reaction with ascorbic acid, during which dehydroascorbic acid and iodide ion are formed.
How do you test for vitamin C with iodine?
Add 3 drops of starch solution to each. Add iodine tincture to each, drop by drop — recording the number of drops — until the iodine no longer reacts and a blue-black color appears. Approximately 1 drop for the water sample compared to 10-15 drops for sample with vitamin C.
How do you calculate the concentration of iodine?
The molecular mass of iodine (I2) is 253.8 g/mol so the number of moles in 2 g is 0.0079 mol (2 g/253.8 g/mol). And the molar concentration of iodine is 0.0079 mol/0.1 L or 0.079 M.
What is the purpose of titrating vitamin C ascorbic acid with the iodine solution?
Vitamin C, more properly called ascorbic acid, is an essential antioxidant needed by the human body (see additional notes). As the iodine is added during the titration, the ascorbic acid is oxidised to dehydroascorbic acid, while the iodine is reduced to iodide ions.
How do you measure vitamin C concentration?
To calculate the amount of vitamin C in 1 test oz., divide the number of mg in the vitamin C tablet by 16 oz. For example, if you use a 1000 mg vitamin C tablet, 1000/16=62.5 mg of vitamin C in every oz. of solution.
What is titration technique?
Titration is a technique where a solution of a known concentration is used to determine the unknown concentration of a second solution. Typically, the titrant (the known solution) is added from a burette to a known quantity of the analyte (the second solution) until the reaction is complete.
How do you make a 20% iodine solution?
Dissolve KI in about 20-30 ml of distilled water. Add iodine and heat gently with constant mixing until iodine is dissolved. Dilute to 100 ml with distilled water. Store in amber glass-stoppered bottle in the dark.