What is C2C12 cell line?
The C2C12 cell line is a subclone of myoblasts that were originally obtained by Yaffe and Saxel at the Weizmann Institute of Science in Israel in 1977. Developed for in vitro studies of myoblasts isolated from the complex interactions of in vivo conditions, C2C12 cells are useful in biomedical research.
Where do C2C12 cells come from?
C2C12 is a subclone from a myoblast line established from normal adult C3H mouse leg muscle1. The original cell line, C2, was obtained by Yaffe and Saxel in 1977 by establishing primary cultures from the thigh muscle of 2 month old normal mice, 70 hours after crush injury2.
What are C2C12 myoblast cells?
C2C12 is a myoblast cell line that is a subclone (produced by H. Blau, et al) of the mouse myoblast cell line established by D. Yaffe and O. Saxel. The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins.
Do C2C12 cells express Pax7?
Rather, Pax7 was strictly expressed in myogenic cells, including low levels in proliferating C2C12 mouse myoblasts, which is a continuous cell line originally derived from satellite cells (Figure 1B).
What is C2C12 differentiation?
Differentiation of C2C12 cells is achieved by replacing GM to differentiation media, DM [DMEM—high glucose no sodium pyruvate (Gibco), 2% horse serum (Gibco), 1% glutamine (Gibco), 1% pen/strep (Gibco)]. After 24 h in DM, fused cells should be visible. DM should be changed every 48 h.
How big are C2C12 cells?
At the time of the biophysical studies, mean myotube diameter was 12 microns (range 5-25 microns), and mean length was 290 microns (range 130-520 microns).
How do C2C12 cells grow?
To propagate the cells, treat C2C12 myoblasts with trypsin. Seed at 1:10 dilution in growth medium into glass-bottom dishes and grow at 37 °C in humidified atmosphere containing 5% CO2 until they reach confluence. 3. Switch the cells from growth to differentiation medium and allow cells to differentiate for 5 days.
What is C2C12 Myotube?
C2C12 myotubes express contractile proteins and, when left to differentiate for an extended period of time, can spontaneously contract. These properties make C2C12 cells an invaluable tool for understanding the molecular biology of muscle development. However, these cells do not perfectly mimic in vivo muscle fibers.
Do myoblasts express Pax7?
Therefore, maintained expression of Pax7 in satellite-cell-derived myoblasts is compatible with differentiation and fusion into multinucleated myotubes. Maintained Pax7 expression delays the onset of myogenin expression.
What is Pax7 gene?
PAX7 Gene – Paired Box 7 This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth.
What happens after the fusion of myoblasts?
Muscle fibers generally form through the fusion of precursor myoblasts into multinucleated fibers called myotubes. In the early development of an embryo, myoblasts can either proliferate, or differentiate into a myotube.