Why is TRIzol pink in Colour?

Why is TRIzol pink in Colour?

The guanidinium salt serves as a chaotropic agent to denature proteins and the phenol (commonly indicated as a pink color) is an organic compound also used to extract nucleic acids and proteins 3,4.

How do you prepare TRIzol reagent?

Method

1. Grind ~100 mg tissue in LN2 in a 10 ml polypropylene tube and a glass rod.
2. Transfer powder to 1.5 mL tube (approx 1/4th of the tube filled with powder)
3. Add 1.2 ml TRIzol (TRIzol is best 1:10 (1 g 10 ml), but 1:5 and 1:2 works also fine)
4. Mix well by inverting, and make sure the mixture is completely molten.

Does TRIzol remove DNA?

TRIzol® reagent is an acid-guanidinium-phenol based reagent ideally designed for the extraction of RNA (as well as DNA and protein) from various biological sample inputs. The low pH (acidic) of TRIzol® controls to separate RNA from DNA and protein, while a high pH can cause RNA and DNA to be isolated together.

What is TE buffer?

TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. “TE” is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+.

What is TRIzol reagent?

TRIzol Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA

Is total RNA isolated by TRIzol® reagent free of protein contamination?

Total RNA isolated by TRIzol® Reagent is free of protein and DNA contamination. TRIzol® Reagent allows you to perform sequential precipitation of RNA, DNA, and proteins from a single sample.

How long does it take to extract RNA from TRIzol?

The simplicity of the TRIzol® Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in 1 hour. Total RNA isolated by TRIzol® Reagent is free of protein and DNA contamination.

What is TRI Reagent®?

Visit Product Comparison Guide TRI reagent® also known as TRIzol, is a monophasic solution of phenol and guanidinium isothiocyanate. It has a potential, to simultaneously solubilize biological material and denatures protein. It is widely used for deproteinizing RNA.

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