What is neutral red stain used for?
Neutral red (toluylene red, Basic Red 5, or C.I. 50040) is a eurhodin dye used for staining in histology. It stains lysosomes red. It is used as a general stain in histology, as a counterstain in combination with other dyes, and for many staining methods.
Is neutral red a neutral stain?
It has a role as a dye, an acid-base indicator and a two-colour indicator. It contains a neutral red(1+). A vital dye used as an indicator and biological stain.
What is neutral red assay?
The neutral red uptake assay is a cell viability assay that allows in vitro quantification of xenobiotic-induced cytotoxicity. The assay relies on the ability of living cells to incorporate and bind neutral red, a weak cationic dye, in lysosomes.
Is neutral red a nuclear stain?
For almost 100 years, neutral red has been used to stain living cells and fixed tissue. It can be used as a general-purpose stain, a pH indicator (turning from red to yellow, as the medium becomes alkaline), or a nuclear stain. In this protocol, neutral red is used to stain plant vacuoles.
Is neutral red acidic or basic?
CHEBI:86370 – neutral red
| ChEBI Name | neutral red |
|---|---|
| Definition | A hydrochloride obtained by combining the free base of neutral red with one equivalent of hydrochloric acid. Neutral red acts as a pH indicator, changing from red to yellow between pH 6.8 and 8.0. |
| Stars | This entity has been manually annotated by the ChEBI Team. |
Is neutral red the same as phenol red?
Chambers and Kempton (’33) found that phenol red is actively secreted, while neutral red accumu- lates within the cells and is not passed into the relatively alkaline fluid within the lumen of the tubules.
Is neutral red a pH indicator?
Neutral red acts as a pH indicator, changing from red to yellow between pH 6.8 and 8.0.
Can neutral red pass through cell membrane?
The neutral red (3-amino-7-dimethyl-amino-2-methylphenazine hydrochloride) is a weak cationic dye that readily penetrates cell membranes by non-ionic diffusion. In intact cells, it binds and accumulates with anionic sites in the lysosome, becomes charged and does not freely pass out into the cytoplasm.
What is the pH of phenol red?
6.2 to 8.2
Phenol red, also known as phenolsulfonphthalein, is a pH indicator dye that exhibits a gradual transition from yellow to red over a pH range of 6.2 to 8.2 (Figure 2). Above 8.2 the dye turns a bright fuchsia color.
At what pH does phenolphthalein turn pink?
pH 8.2
Phenolphthalein is naturally colourless and works differently then litmus paper. Also, it turns in pink colour in an alkaline solution or base. Moreover, the compound remains colourless in acids but starts to turn pink on pH 8.2 and continues to turn bright purple in strong bases.
What color is phenol red in a neutral solution?
A solution of phenol red is used as a pH indicator, often in cell culture. Its color exhibits a gradual transition from yellow (λmax = 443 nm) to red (λmax = 570 nm) over the pH range 6.8 to 8.2. Above pH 8.2, phenol red turns a bright pink (fuchsia) color. and is orange-red.