What is the purpose of 5 RACE?

What is the purpose of 5 RACE?

The 5′ RACE System is a set of prequalified reagents intended for synthesis of first strand cDNA, purification of first strand products, homopolymeric tailing, and preparation of target cDNA for subsequent amplification by PCR.

What is the purpose of 5 race?

What is a 5 race?

How long is the annealing step in PCR?

30 sec to 1 min
The annealing step (30 sec to 1 min, at temperatures 45–60 °C), is required so that the primers bind to the complementary sequence on each of the DNA single strands. The primers are designed such that they bracket the target of interest and the region of sequence that lies between them is referred to as the amplicon.

What is 5’RACE-PCR or anchored PCR?

RACE can provide the sequence of an RNA transcript from a small known sequence within the transcript to the 5′ end (5′ RACE-PCR) or 3′ end (3′ RACE-PCR) of the RNA. This technique is sometimes called one-sided PCR or anchored PCR .

Which cDNAs are ready for 5′-and 3′-RACE PCR?

These cDNAs are ready for 5′- and 3′-RACE PCR and are available from a wide range of tissues and cell types. Cat. # This kit contains reagents for making adaptor-ligated ds cDNA from poly A+ RNA and performing 5′ and 3′ Marathon RACE PCR using gene-specific primers and a mixture of thermostable DNA polymerases supplied by the user.

What is the 5 race system used for?

Summary of the 5′ RACE System. The 5′ RACE System is a set of prequalified reagents intended for synthesis of first strand cDNA, purification of first strand products, homopolymeric tailing, and preparation of target cDNA for subsequent amplification by PCR.

What primers are required for 5′ race?

A minimum of two antisense gene-specific primers (GSP) are required for 5′ RACE and must be supplied by the user. GSP1 primes first-strand cDNA synthesis. A second nested primer, GSP2, that anneals to sequences located 3′ (with respect to cDNA not mRNA) of GSP1 is required for PCR.