What is the principle of atomic absorption spectrophotometer?
AAS is an analytical technique used to determine how much of certain elements are in a sample. It uses the principle that atoms (and ions) can absorb light at a specific, unique wavelength. When this specific wavelength of light is provided, the energy (light) is absorbed by the atom.
What are the applications of atomic absorption spectroscopy?
Atomic absorption spectroscopy is utilized across many industries and is instrumental in the detection of metals within a sample. As such, this process is commonly utilized in pharmacology, archaeology, manufacturing, mining, and forensics.
What is the instrumentation of atomic absorption spectroscopy?
Instrumentation used to carry out atomic absorption spectrophotometry requires a source of light that matches the narrow bands of light that a particular atom absorbs (a hollow cathode lamp), a flame or graphite furnace to heat the sample, a monochromator to select the wavelength of light, and a photodetector.
What are the limitations of atomic absorption spectroscopy?
Background correction
| Advantages | Limitations |
|---|---|
| Low cost per analysis | Cannot detect non-metals |
| Easy to operate | New equipment is quite expensive |
| High sensitivity (up to ppb detection) | More geared towards analysis of liquids |
| High accuracy | Sample is destroyed |
What is the function of flame in atomic absorption spectrophotometry?
The flame is lined up in a beam of light of the appropriate wavelength. The flame (thermal energy) causes the atom to undergo a transition from the ground state to the first excited state. When the atoms make their transition, they absorb some of the light from the beam.
What are the advantages of using atomic absorption spectrophotometry?
The main advantages of AAS are that it is relatively inexpensive and easy to use, while still offering high throughput, quantitative analysis of the metal content of solids or liquids. This makes it suitable for use in a wide range of applications.
How to calculate atomic absorption spectroscopy?
The student will analyze a sample of iron ore using atomic absorption spectrophotometry.
What is the principle of atomic absorption spectroscopy?
Quantitative metal concentrations in solution
How does an atomic absorption spectrometer work?
A light source
What can atomic absorption spectroscopy be used for?
Atomic absorption spectroscopy is based on absorption of light by free metallic ions. In analytical chemistry the technique is used for determining the concentration of a particular element (the analyte) in a sample to be analyzed.