What is the chloramphenicol resistance gene?
The chloramphenicol resistance gene (pp-cat) was cloned from a transferable R plasmid of Pasteurella piscicida, pSP9351, and the sequence of the gene was determined. Subcloning and deletion analysis localized the resistance gene, pp-cat, to within a 2.3 kb HincII-BamHI fragment.
What are the enzymes encoded by the chloramphenicol resistance genes?
The primary mechanism of resistance to chloramphenicol is due to the presence of chloramphenicol acetyltransferase (CAT), an enzyme which catalyzes the acetylCoA-dependent acetylation of the antibiotic at the C-3 hydroxyl group (58).
What is chloramphenicol acetyltransferase gene?
Chloramphenicol acetyltransferase (CAT) is the classic example among reporter genes. The enzyme catalyzes the transfer of an acetyl remnant of acetyl CoA to chloramphenicol. The cell lysate to be examined is incubated with acetyl CoA and [14C]-labeled chloramphenicol.
What does CMR gene do?
A novel gene designated cmr, which mapped to 18.8 min of the Escherichia coli K-12 genome, was shown to mediate resistance to chloramphenicol when it was expressed from a multicopy vector.
How do bacteria acquire resistance genes?
Bacteria can acquire antibiotic resistance genes from other bacteria in several ways. By undergoing a simple mating process called “conjugation,” bacteria can transfer genetic material, including genes encoding resistance to antibiotics (found on plasmids and transposons) from one bacterium to another.
What is the cellular target of chloramphenicol?
Chloramphenicol stops bacterial growth by binding to the bacterial ribosome (blocking peptidyl transferase) and inhibiting protein synthesis. Chloramphenicol is lipid-soluble, allowing it to diffuse through the bacterial cell membrane.
Where is the chloramphenicol resistance gene located in Rhodococcus fascians?
The nucleotide sequence of the chloramphenicol-resistance gene (cmr) of Rhodococcus fascians NCPPB 1675 (located on the conjugative plasmid pRF2) allowed the identification of two possible open reading frames (ORFs), of which ORF1 was consistent with the mutational analysis.
How is chloramphenicol resistance gene (pp-Cat) made?
The chloramphenicol resistance gene (pp-cat) was cloned from a transferable R plasmid of Pasteurella piscicida, pSP9351, and the sequence of the gene was determined. Subcloning and deletion analysis localized the resistance gene, pp-cat, to within a 2.3 kb HincII-BamHI fragment. The fragment as a pr …
What is the pathophysiology of chloramphenicol-resistant strains from outbreaks?
A feature of chloramphenicol-resistant strains from such outbreaks was that although the strains belonged to different Vi PTs, resistance to chloramphenicol – often in combination with resistance to streptomycin, sulfonamides, and tetracyclines (R-type CSSuT) – was encoded by a plasmid of the H1 incompatibility group (now termed HI1).
What is the size of the element chloramphenicol resistance?
Chloramphenicol resistance could be readily lost during conjugative transfer of Tn4451 and the size of the element was determined to be approximately 6 kb from restriction endonuclease analysis of deletion derivatives (Abraham and Rood, 1987; ii. Use of CAT gene as selectable marker.