How does Fluidigm work?

How does Fluidigm work?

The Fluidigm single-cell targeted DNA sequencing workflow gives you the power to identify somatic mutations in known genes or loci from cell populations that are undetectable in bulk samples.

What is Fluidigm C1?

The innovative C1 system carefully isolates single cells into individual reaction chambers in the exclusive Fluidigm integrated fluidic circuit (IFC). The optically clear IFC lets you automatically stain captured cells and examine them by microscopy for viability, surface markers or reporter genes.

What is smart Seq2?

Method Category: Transcriptome > RNA Low-Level Detection. Description: For Smart-Seq2, single cells are lysed in a buffer that contains free dNTPs and oligo(dT)-tailed oligonucleotides with a universal 5′-anchor sequence. RT is performed, which adds 2–5 untemplated nucleotides to the cDNA 3′ end.

What is drop seq?

Description: Drop-Seq analyzes mRNA transcripts from droplets of individual cells in a highly parallel fashion. This single-cell sequencing method uses a microfluidic device to compartmentalize droplets containing a single cell, lysis buffer, and a microbead covered with barcoded primers.

What is smart seq?

Smart-Seq and Smart-Seq2: Switch Mechanism at the 5′ End of RNA Templates Smart-Seq was developed as a single-cell sequencing protocol with improved read coverage across transcripts. Complete coverage across the genome allows the detection of alternative transcript isoforms and SNPs.

How does 10X Genomics work?

10x Genomics’ single-cell RNA-seq (scRNA-seq) technology, the Chromium™ Single Cell 3′ Solution, allows you to analyze transcriptomes on a cell-by-cell basis through the use of microfluidic partitioning to capture single cells and prepare barcoded, next-generation sequencing (NGS) cDNA libraries.

What are barcoded beads?

Barcoded magnetic beads are immunomagnetic beads combined with “digital barcodes” say oligonucleotides to create a new, bio-inspired magnetic beads.