How are oligosaccharides synthesized?
The chemical synthesis of oligosaccharides usually involves reactions between saccharide derivatives that possess good leaving groups at the anomeric position, such as a halogen atom or an ester group, and an adduct, which may be a monosaccharide or an oligosaccharide.
What is Merrifield solid phase synthesis?
The synthesis of peptides and small proteins in which the resinous polymer supported amino acid and succeeding peptide repeatedly reacts with N-protected amino acids followed by deprotection until the desired peptide or protein is assembled is generally referred to as the Merrifield solid phase peptide synthesis (or …
What is the first step in the solid phase peptide synthesis?
Introduction. Solid-phase peptide synthesis (SPPS) involves the successive addition of protected amino acid derivatives to a growing peptide chain immobilized on a solid phase, including deprotection and washing steps to remove unreacted groups and also side products.
What is the structure of oligosaccharides?
Oligosaccharide is a carbohydrate polymers comprise three to ten monosaccharides, or, simple sugars. They were linked together mostly by O-glycosidic bond through condensation reaction between an anomeric carbon of a monosaccharide and the other. They can also form N-glycosidic linkages under certain atmosphere.
What is solid phase peptide synthesis used for?
The concept of solid-phase peptide synthesis (SPPS) is to retain chemistry that has been proven in solution but to add a covalent attachment step that links the nascent peptide chain to an insoluble polymeric support (resin).
Who invented solid phase peptide synthesis?
Bruce Merrifield
To this day, Bruce Merrifield is still considered the father of modern peptide synthesis. It was Merrifield who revolutionized this process by developing a solid-phase technique in the 1960s.
Which is a main advantage of solid phase synthesis?
General advantages of solid phase synthesis are easy purification, rapid generation of linear peptide intermediates, and precedent in the synthesis of large peptides.
How to synthesize oligosaccharides on solid-phase synthesis?
We describe the automated chemical synthesis of several oligosaccharides on a solid-phase synthesizer. A branched dodecasaccharide was synthesized through the use of glycosyl phosphate building blocks and an octenediol functionalized resin. The target oligosaccharide was readily obtained after cleavage from the solid support.
How to synthesize dodecasaccharide from glycosyl phosphate?
A branched dodecasaccharide was synthesized through the use of glycosyl phosphate building blocks and an octenediol functionalized resin. The target oligosaccharide was readily obtained after cleavage from the solid support.
What is solid phase synthesis?
Solid phase synthesis was first established for oligopeptides and provided the basis for automated peptide synthesis ( Merrifield, 1985 ). Solid phase synthesis was later extended to the synthesis of oligonucleotides ( Caruthers, 1985; Matteucci and Caruthers, 1981 ).
What are oligosaccharides and why are they important?
Oligosaccharides are an important class of biomolecules, and are involved in many biochemical processes ( Apweiler et al., 1999, Paulson, 1989, Varki et al., 1999 ). In higher organisms, certain proteins are posttranslationally modified by linking various oligosaccharides to amino acid side chains to form glycoproteins.