What is Immunoturbidimetry technique?

What is Immunoturbidimetry technique?

Immunoturbidimetry. Immunoturbidimetry is an important tool in the broad diagnostic field of clinical chemistry. It is used to determine serum proteins not detectable with classical clinical chemistry methods. Immunoturbidimetry uses the classical antigen-antibody reaction.

What is the meaning of Turbidimetry?

[ tûr′bĭ-dĭm′ĭ-trē ] n. A method for determining the concentration of a substance in a solution by measuring the loss in intensity of a light beam through a solution that contains suspended particulate matter.

What is the principle of the turbidimetric tests?

In turbidimetry, an incident beam of visible light is passed through a sample in a cuvette, and the intensity of the transmitted light is then measured. As Ag:Ab complexes form, light is increasingly scattered away from the incident light path, resulting in a reduction in transmitted light intensity.

What is turbidimetric method in microbiology?

The turbidimetric method is characterized by a series of tubes containing different concentrations of antibiotics in a liquid culture medium inoculated with the test microorganism. Reading data obtained after incubation provides a curve displaying the antibiotic concentration with turbidity.

What is Immunoturbidimetry normal range?

The normal range is usually 11 to 69 ng/ml.

How does a Nephelometer work?

The nephelometer is an instrument that measures aerosol light scattering. It detects scattering properties by measuring light scattered by the aerosol and subtracting light scattered by the gas, the walls of the instrument and the background noise in the detector.

What is Immunonephelometry technique?

Immunonephelometry is a related method where reflected rather than absorbed light is measured, which aids in reducing interference. Another modification of the IT test is to make use of antibody-coated latex particles, which can make assays more sensitive as well as stabilizing the antibodies.