What is silica gel 60 gf254?
In TLC, silica gel 60 sorbent is considered the most versatile. Our assortment of silica gel 60 features different grades with particle sizes ranging from 5-40 µm: silica with a gypsum binder, silica with no foreign binder, and silica gel with a fluorescence indicator.
What does F254 mean?
Jack Silver. Teledyne ISCO, Inc. Silica with F254 has an flourescent material that absorbs light at 254 nm (hence the 254 name) and emits in the visible spectrum.
Is silica TLC plate polar?
Silica gel is simply very finely ground very pure sand. It should be noted that silica gel is highly polar and is capable of hydrogen bonding. Consider the side-on view of the development of a TLC plate below.
What is the thickness of TLC plate?
The layer thickness is related to the nature of the analysis (analytical or preparative) as well as the performance of the plate (TLC or HPLTC). The most common layer thicknesses are 150 µm (HPTLC plates), 200 – 250 µm (analytical TLC plates) and 500 – 2,000 µm (preparative TLC plates).
What is TLC plate made of?
The thin-layer chromatography plate is composed of silica gel (the adsorbent ) adhered to a plastic backing for support. Reminder: silica is a polar compound.
Does silica gel react with anything?
Silica gel is non-toxic, non-flammable, and non-reactive and stable with ordinary usage. It will react with hydrogen fluoride, fluorine, oxygen difluoride, chlorine trifluoride, strong acids, strong bases, and oxidizers.
What does G stand for in Silica Gel G?
Gypsum
The “G” stands for Gypsum and indicates that calcium sulfate hemihydrate (CaSO4·1/2H2O), a refined form of gypsum, has been included in the adsorption formulation. Gypsum functions as a binder, improving the cohesion of the adsorbent particles and increasing the adhesion of the layer to the glass substrate.
Does Silica Gel react with anything?
What happens if the TLC solvent is too polar?
If your eluent(s) is very polar relative to your compound, it will dissolve your sample and carry it in the mobile phase. Overall, the eluent solvent(s) (also called the mobile phase) will compete with your sample for a space on the adsorbent (stationary phase) coated on the TLC plate.
What is the main reason that silica gel is so polar?
Silica gel, the most commonly used stationary phase, has the empirical formula SiO2. However, at the surface of the silica gel particles, the dangling oxygen atoms are bound to protons. The presence of these hydroxyl groups renders the surface of silica gel highly polar.
How many types of TLC are there?
SiliCycle offers different types of plates for thin layer chromatography applications: classical TLC, high performance TLC (also called HPTLC) and preparative TLC (PLC). The plate types are selected based on the type of analysis required and the available budget.
What is the length of a TLC plate?
Thin layer chromatography (TLC) is used frequently to visualize components of a mixture. The most common TLC plate is typically a rectangular piece of glass (2.5 cm x 7.5 cm) coated with silica powder.